Figure 3.

Detection of transient interactions. (a) Each circle shows the percentage of protein-pairs in a specific set/complex with a significant level (P-value < 0.05) of S. cerevisiae and orthologous co-expression in the horizontal and vertical axes, respectively. Blue circles represent all pair-wise interactions in a single transient complex; Red circles represent the three sets of protein-pairs (random, reference and transient). The dashed line indicates similar performance of both methods. The table also shows the number of protein-pairs in each set/complex, and the ratio between the percentage of pairs with significant orthologous and S. cerevisiae co-expression, respectively. (b) Proposed model for transient yeast interactions with low co-expression, but high orthologous co-expression. Protein A interacts with protein B, but also performs other functions or interacts with other proteins, such that it is not co-expressed with protein B. However, in higher eukaryotes, a specialized ortholog of A exist, which is co-expressed with the ortholog of B.