Three-panel diagnostic summary The standard curve panel (A) of the three-panel diagnostic summary features a scatterplot of the data, the estimated standard curve (black line), the 95% confidence intervals (blue lines), and the region of acceptable errors (grey). For this example, the acceptable segment of the standard curve (i.e., the segment with concentration estimation errors acceptable to us) covers spot intensities from about 1000 to 7500 intensity units and concentrations from about 25 to 500 pg/ml. The histogram of sample intensities (B) suggests that about one-fifth of the sample spot intensities is below the detection limit of about 1000 units, about three-fifths will produce estimated concentrations in the acceptable range, and one-fifth exceeds the acceptable range. With regard to remeasuring the standards in this experiment or imaging in future experiments, the three-panel graph suggests that it may be worthwhile to attempt to extend the acceptable range. It also suggests that the normalization between the standards measurements and sample measurements should be revisited. The graph of the concentration coefficient of variation as function of concentration (C) offers an alternative summary of the estimation errors over the range of concentrations.
Daly et al. BMC Bioinformatics 2005 6:17 doi:10.1186/1471-2105-6-17