Screenshot from the Artemis sequence viewer  showing a sample region of D. radiodurans and accompanying RescueNet predictions. Annotated genes are shown as white blocks, and predictions are shown in-frame as shaded blocks. Note the relative infrequency of stop codons (vertical lines in each frame) and the many ORFs that are not protein-coding regions. Note also the selected gene DR1142 and the contradicting RescueNet prediction. DR1142 is a hypothetical gene, predicted to be so by Glimmer2, and there is a strong possibility that the CDS marked by RescueNet is the correct prediction. The possibility is also raised by RescueNet that the gene DR1143 may be longer than previously annotated and contains a frameshift.
Mahony et al. BMC Bioinformatics 2004 5:23 doi:10.1186/1471-2105-5-23