Table 2

Sequences used for the negative control

Protein

Host organism

Evidence for non-allergenicity

Accession

Reference

Amaranth seed albumin

Amaranthus hypochondriacus

IgG-response, but no raised IgE-levels, after administration (intranasal and intraperitoneal) of amaranth seed albumin to mice

GenPept CAA77664

[14]

T1

Catharanthus roseus

No reaction of recombinant T1 in IgE-sera binding, basophile histamine release, and skin prick testing using patients allergic to the related birch pollen allergen Bet v 1

Not applicable

[15]

Mite ferritin heavy chain

Dermatophagoides pteronyssinus

Reaction of mite ferritin with IgG, but not with IgE, of sera from patients allergic to house dust mite

GenPept AAG02250

[16]

Maltose binding protein

Escherichia coli

No reaction with IgE-sera from patients allergic to natural rubber latex (maltose binding protein used as part of fusion proteins with latex allergens)

SwissProt P02928

[17]

Human serum albumin

Homo sapiens

No reaction of human serum albumin with IgE-sera of patients allergic to cat- and porcine-serum albumin

SwissProt P02768

[18]

Human heat shock protein 70

Homo sapiens

No reaction of human heat shock protein 70 with IgE-sera of patients allergic to heat shock protein 70 from Echinococcus granulosus

SwissProt P08107

[19]

Human beta-2-glycoprotein I

Homo sapiens

Presence of IgM antibodies, but not of IgE antibodies, directed against human beta-2-glycoprotein I in sera from atopic eczema/dermatitis patients

SwissProt P02749

[20]

Guayule rubber particle protein

Parthenium argentatum

No cross-reactivity between proteins from guayule and latex using IgE-sera from patients allergic to latex

Swissprot Q40778

[21]

Purple acid phosphatase 1

Solanum tuberosum

Stimulation of IgG-, but no or only low stimulation of IgE-antibodies following administration of potato acid phosphatase to mice (oral and intraperitoneal)

TrEMBL Q6J5M7

[22]

Purple acid phosphatase 2

Solanum tuberosum

See above

TrEMBL Q6J5M9

[22]

Purple acid phosphatase 3

Solanum tuberosum

See above

TrEMBL Q6J5M8

[22]

Potato lectin

Solanum tuberosum

Stimulation of IgG-, but no or only low stimulation of IgE-antibodies following administration of potato lectin to mice (intraperitoneal)

TrEMBL Q9S8M0

[23]

Fiers et al. BMC Bioinformatics 2004 5:133   doi:10.1186/1471-2105-5-133