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Unique coding sequences in CELO and related avian adenoviruses |
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| CELO-ORFa |
Regionb |
Strand |
Length (aa) |
Homologous sequencesc |
Comment |
|
|
|
|
|
|
|
| ORF-1 |
794–1330 |
forward |
178 |
FadV-9-ORF847–1338 |
functional dUTP pyrophosphatase [4], homologous to ORF-1 proteins of mastadenovirus E4 region [5] |
| ORF-2 |
1999–2829 |
forward |
276 |
FadV-9-ORF1950–2753 |
|
| ORF-8 |
37391–38239 |
forward |
282 |
FadV-9-ORF37859–38668 FadV-10-ORF2147–2911 |
Gam1, antiapoptotic, induction of heat shock response, inactivation of histone deacetylase 1, pRb/E2F pathway [6-10] |
| ORF-9 |
40037–41002 |
forward |
321 |
FadV-9-ORF43595–42660 CFA40-ORF17739-16381 EST-ORF-9 |
amino terminus unclear, since transcript was shown to be spliced up to 40133 [13] |
| ORF-10 |
41002–41853 |
forward |
283 |
no homologs |
Translation start is likely to be at pos. 41113 |
| ORF-11 |
41958–42365 |
forward |
135 |
FadV-9-ORF41461–41853 |
|
| ORF-12 |
5412-4462 |
reverse |
315 |
FadV-9-ORF6190-5243 |
amino terminus extended by 106 residues |
| ORF-13 |
4568-3549 |
reverse |
339 |
FadV-9-ORF5058-4261 |
|
| ORF-14 |
3503-2892 |
reverse |
203 |
FadV-9-ORF4180-3536 FadV-9-ORF3412-2837 |
amino terminus extended by 43 residues |
| ORF-16 |
39705-39286 |
reverse |
139 |
FadV-10-ORF4550-4209 |
|
| ORF-17 |
39256-38717 |
reverse |
179 |
FadV-9-ORF41096-40596 |
|
| CFA40-ORF15112-14642 |
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| FadV-10-ORF4023-3574 |
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| ORF-18/19 |
(36144)-34238 |
reverse |
635 |
FadV-9-ORF36385-34220 CFA40-ORF10653-8782 FadV-10-ORF4992-5864 FadV-10-ORF6050-7456 |
Correction of an obvious error in the genomic sequence combines ORF-18 and ORF-19 to one single ORF. The amino terminus was extended by the translation of a short leader sequence that was shown to be spliced ahead of the original ORF-18 [13]. |
| ORF-20 |
33832-32892 |
reverse |
313 |
FadV-9-ORF33963-32986 CFA40-ORF8466-7741 |
amino terminus extended by 42 residues |
| ORF-22 |
32429-31812 |
reverse |
205 |
FadV-9-ORF32502-31930 CFA40-ORF3306-2729 |
involved in pRb/E2F pathway [10] |
| ORF28115-27765 |
28115-27765 |
reverse |
117 |
FadV-9-ORF30192-29797 CFA40-ORF4777-4478 FadV-10-ORF1814-1637 |
New conserved ORF, located between fibre and pVIII gene |
| ORF32895-32434 |
32895-32434 |
reverse |
154 |
FadV-9-ORF32985-32509 CFA40-ORF7552-7262 |
new conserved ORF, is translation of the original ORF-21 in a different frame |
|
a ORF-numbering in accordance to Chiocca et al. [4]b coordinates in the genomic sequence of CELO (acc.no. NC_001720) c ORFs are named by their coordinates in the following nucleic acid sequences: NC_000899 (FAdV-9), AF155911 (CFA40) and AF160185 (FAdV-10). FAdV-10-ORF1814-1637 is derived from entry AF006739, CFA40-ORF3306-2729 from entry U40587. EST-ORF-9 is the translation of an EST sequence (BM491231) of a chicken EST library. | |||||
Washietl and Eisenhaber BMC Bioinformatics 2003 4:55 doi:10.1186/1471-2105-4-55 |
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