Table 2 

List of Normalization Methods 

List of normalization methods including printtip scale normalization 



Method 
Notation 
Description 


O 
Original data 



Global 
G 
Global median normalization 
GP 
Global median normalization on each printtip 

GPS 
Global median normalization on each printtip with scale normalization 

G.s 
Global median normalization and betweenslide scale normalization 

GP.s 
Global median normalization on each printtip and betweenslide scale normalization 

GPS.s 
Global median normalization on printtip with scale normalization and betweenslide scale normalization 



Linear 
L 
Intensity dependent linear regression normalization 
LP 
Intensity dependent linear regression normalization on each printtip 

LPS 
Intensity dependent linear regression normalization on each printtip with scale normalization 

L.s 
Intensity dependent linear regression normalization and betweenslide scale normalization 

LP.s 
Intensity dependent linear regression normalization on each printtip and betweenslide scale normalization 

LPS.s 
Intensity dependent linear regression normalization on each printtip with scale normalization and betweenslide scale normalization 



Nonlinear 
N 
Intensity dependent nonlinear regression normalization (LOWESS) 
NP 
Intensity dependent nonlinear regression normalization (LOWESS) on each printtip 

NPS 
Intensity dependent nonlinear regression normalization (LOWESS) on each printtip with scale normalization 

N.s 
Intensity dependent nonlinear regression normalization (LOWESS) and betweenslide scale normalization 

NP.s 
Intensity dependent nonlinear regression normalization (LOWESS) on each printtip and betweenslide scale normalization 

NPS.s 
Intensity dependent nonlinear regression normalization (LOWESS) on each printtip with scale normalization and betweenslide scale normalization 



Park et al. BMC Bioinformatics 2003 4:33 doi:10.1186/14712105433 