Table 1

Results of S-S bond determination on a set of Glycosyltransferases using MS-based and sequence-based methods.

Swiss-Prot ID

Known S-S bonds

Methods that detected the bond

Methodology


Q92187

142-292

MassMatrix

MS-based

156-356

MassMatrix

MS-based

P02754

82-176

MassMatrix, DisLocate, DiANNA 1.1

MS-based, Sequence-based

122-135

-

-

Q11130

68-176

MassMatrix, PreCys

MS-based, Sequence-based (cysteine separation profile)

211-214

MassMatrix, PreCys

As above

318-321

MassMatrix, PreCys

As above

P08037

134-176

MassMatrix

MS-based

247-266

MassMatrix

MS-based

Q09324

59-413

-

-

100-172

-

-

151-199

-

-

372-381

-

-

P00698

24-145

DISULFIND, DiANNA 1.1

Sequence-based

48-133

MassMatrix, DiANNA 1.1, DISULFIND

MS-based, Sequence-based

P21217

81-338

-

-

91-341

-

-


The molecules and their Swiss-Prot ID are: ST8Sia IV [Swiss-Prot:Q92187], Beta-lactoglobulin [Swiss-Prot:P02754], FucT VII [Swiss-Prot:Q11130], C2GnT-I [Swiss-Prot:Q09324], Lysozyme [Swiss-Prot:P00698], FT III [Swiss-Prot:P21217], β1-4GalT [Swiss-Prot:P08037], Aldolase [Swiss-Prot:P00883], and Aspa [Swiss-Prot:Q9R1T5]. The methods investigated include MassMatrix [21], DisLocate [24], DISULFIND [14], PreCys [4], and DiANNA 1.1 [17].

Singh and Murad BMC Bioinformatics 2013 14(Suppl 2):S20   doi:10.1186/1471-2105-14-S2-S20

Open Data