Table 2

Estimated parameter values and confidence intervals
Parameter Value 95% Confidence Units
PTS kcat 20.7 ±9.1 mmol/mg protein/hr
PGI kcat 40.2 ±9.5 mmol/mg protein/hr
Keq 1.23 ±0.29 Dimensionless
PFK kcat 26 ±35 mmol/mg protein/mM/hr
ALDO kcat 3.965 ±0.010 mmol/mg protein/mM/hr
Keq 0.18 ±0.17 mM
Kfdp 0.0074 ±0.0036 mM
TPI kcat 10000 ±14000 mmol/mg protein/hr
Keq 0.11400 ±0.00031 Dimensionless
GAPD kcat 10000 ±4100 mmol/mg protein/mM2/hr
Keq 1.21 ±0.14 Dimensionless
PGM kcat 9995 ±40 mmol/mg protein/hr
Keq 0.53570 ±0.00063 Dimensionless
PGK kcat 54.3 ±2.9 mmol/mg protein/hr
Keq 5512.1 ±1.2 Dimensionless
ENO kcat 2 ±45 mmol/mg protein/mM/hr
Keq 1.4 ±4.1 Dimensionless
PYK kcat 40 ±49 mmol/mg protein/hr
PDH Kcat 10.4 ±4.6 mmol/mg protein/hr
Kpyr 0.000020 ±0.000029 mM4
PPC kcat 2.15 ±1.80 mmol/mg protein/hr
Kfdp 2.5 ±6.5 mM
Kpep 0.10 ±0.16 mM
G6PDH kcat 859.6 ±1.1 mmol/mg protein/mM2/hr
GND kcat 18.5 ±10.8 mmol*mM/mg protein/hr
K6pg 0.021 ±0.012 mM
RPI kcat 549.46 ±0.69 mmol/mg protein/mM/hr
Keq 1.40000 ±0.00019 Dimensionless
RPE kcat 10000 ±13000 mmol/mg protein/mM/hr
Keq 0.4900 ±0.0044 Dimensionless
TKT1 kcat 10000 ±7800 mmol/mg protein/mM2/hr
Keq 1.99 ±0.012 Dimensionless
TKT2 kcat 10000 ±5800 mmol/mg protein/mM2/hr
Keq 3.500 ±0.013 Dimensionless
TALA kcat 10000 ±2300 mmol/mg protein/mM2/hr
Keq 0.3675 ±0.0021 Dimensionless

Binding coefficient parameters have units of mM, except for Kpyr in PDH, which has units of mM4. Equilibrium constants (Keq) are dimensionless, except for ALDO, which has units of mM. Values for kcat have units such that fluxes have units of mmol/gDW/h. Global upper and lower bounds for binding and enzyme activity were 104 and 10-6, respectively. Bounds on equilibrium constants were 0.35Keqo and 2.85Keqo, where Keqo is the measured equilibrium constant.

Cotten and Reed

Cotten and Reed BMC Bioinformatics 2013 14:32   doi:10.1186/1471-2105-14-32

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