Table 2

The rating system
Feature Criterion Points
True False NA
Reciprocal BLAST Query-to-hit-genome1 best hit +1 0 +0.253
Hit-to-query-genome2 best hit +1 0 +0.253
Multiple Sequence Comparison (MSC) Method4 Accept with identity cutoff 60% +0.5 0 \
Accept with identity cutoff 50% +0.5 0 \
Accept with identity cutoff 40% +0.5 0 \
Pairwise comparison to the query4 Identity>60% +0.5 0 \
Identity>50% +0.5 0 \
Identity>40% +0.5 0 \
others Reference proteins +1 0 \

1: use the input sequence to BLAST against the genome of the hit.

2: use the hit to BLAST against the genome of the input sequence.

3: the whole genome sequences of the protein are unavailable.

4: coverage in both the input sequence and the hits must larger than 80%.

Li et al.

Li et al. BMC Bioinformatics 2013 14:30   doi:10.1186/1471-2105-14-30

Open Data