Figure 5.

Example output web page from PrimerDesign. The output includes a list of the design parameters specified by the user, a graphical illustration showing primer locations (arrows), the ROI (in grey), and curves for entropy, complexity and Tm, followed by a table of the primer constructs. The output also includes buttons to download the graph in different formats, the primer constructs in tab-delimited format, and the dimerization risk-testing log. This figure shows only the primer graph component, because the table may be very long (depending mainly on how many bio-barcodes were requested).

Brodin et al. BMC Bioinformatics 2013 14:255   doi:10.1186/1471-2105-14-255
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