Example output web page from PrimerDesign. The output includes a list of the design parameters specified by the user, a graphical illustration showing primer locations (arrows), the ROI (in grey), and curves for entropy, complexity and Tm, followed by a table of the primer constructs. The output also includes buttons to download the graph in different formats, the primer constructs in tab-delimited format, and the dimerization risk-testing log. This figure shows only the primer graph component, because the table may be very long (depending mainly on how many bio-barcodes were requested).
Brodin et al. BMC Bioinformatics 2013 14:255 doi:10.1186/1471-2105-14-255