Illustration of initial segmentation.(a) The original image. (b) The segmentation ground truth. (c) The interest regions detected without iterative contrast enhancement, and darker blue denotes upper-level regions. (d) The image after iterative enhancement. (e) The final interest regions detected. After the initial segmentation, decluster processing is performed, with outputs shown in (f) and dark gray indicating the detected cell nuclei.
Song et al. BMC Bioinformatics 2013 14:173 doi:10.1186/1471-2105-14-173