Overview of the plankton analysis. During the automated microscopy bright field and fluorescence pictures are taken for different positions in the Utermöhl chamber. For the image analysis all particles are segmented from the background of the bright field image and features are calculated. After manual sorting, the segmented images can be used to train a neural network which is then able to classify new images according to taxon.
Schulze et al. BMC Bioinformatics 2013 14:115 doi:10.1186/1471-2105-14-115