In vivo activity of Mojo Hand-designed TALEN. a) Detail design of TALEN binding site targeting zebrafish flt3 Exon 1 sequence. b) Description and results of the RFLP assay showing targeted small deletion (loss of NsiI recognition sequence) introduced by the Mojo Hand-designed TALEN pair. Primers for RFLP assay: Forward-TGAAAGTCTTCTTGCCTCTGTTC; Reverse- CAGCTGTAAATGAGTCTCACAGTT.
Neff et al. BMC Bioinformatics 2013 14:1 doi:10.1186/1471-2105-14-1