Evidence of risk of coamplification due to NumtS. Chimpanzee NumtS spanning within mitochondrial region from 1006 to 1771; first and second large blocks in red and blue include NumtS on minus and plus strand respectively. Red squared NumtS IDs (Ptr_NumtS _443_b1 and Ptr_NumtS _376_b1) correspond to potentially unintended nuclear regions found by Primer-Blast software, and have fewest mismatches. Red squared NumtS Ptr_NumtS _442_b1 not found by Primer-Blast software, has more mismatches.
Calabrese et al. BMC Bioinformatics 2012 13(Suppl 4):S15 doi:10.1186/1471-2105-13-S4-S15