Table 1

Strains, plasmids and biological devices used in this work.

Strains


Name

Genotype

Source


DB3.1

F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20 <a onClick="popup('http://www.biomedcentral.com/1471-2105/13/S4/S11/mathml/M15','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2105/13/S4/S11/mathml/M15">View MathML</a> ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1

Invitrogen

BW23474

F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510

CGSC, Yale University, USA

TOP10

F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

Invitrogen

MG1655

F- λ-ilvG- rfb-50 rph-1

CGSC Yale University, USA

MG-HSLRFP

MG1655, Φ80(HSLRFP )

This study

MG-101RFP

MG1655, Φ80(101RFP)

This study

MG-IQRFP

MG1655, Φ80(IQRFP)

This study


Vector backbones used for RFP expression or genomic integration of the devices


Name

Replication origin

BioBrick™vector code

Antibiotic resistance


pHC

pUC19-derived pMB1 origin (high copy)

pSB1A2

Ampicillin

pMC

pMR101-derived p15A origin (medium copy)

pSB3K3

Kanamycin

pLC

pSC101 origin (low copy)

pSB4C5

Chloramphenicol

pΦ80

R6K conditional origin, used for integration

BBa_K300000

Chloramphenicol


Genetic devices


Name

Description


HSLRFP

HSL-inducible mRFP1 expression system

HSLGFP

HSL-inducible GFPmut3b expression system

101RFP

Standard reference constitutive promoter with mRFP1 expression device downstream

101GFP

Standard reference constitutive promoter with GFPmut3b expression device downstream

IQRFP

lacIQ constitutive promoter with mRFP1 expression device downstream


Zucca et al. BMC Bioinformatics 2012 13(Suppl 4):S11   doi:10.1186/1471-2105-13-S4-S11

Open Data