Characterization of the lacIQ constitutive promoter in RPUs. The ratio between the activities (Scell) of lacIQ and J23101 promoters, measured via IQRFP and 101RFP devices respectively, was computed to obtain the lacIQ RPUs in the four copy number conditions investigated in this work. Consider that IQRFP and 101RFP have a different DNA scar between promoter and RBS. Unpublished data from our lab showed that the scar present in IQRFP systematically overestimates promoters activity by 1.43-fold when compared to the scar in 101RFP. Error bars represent the 95% confidence intervals of the mean value computed on 3 clones.
Zucca et al. BMC Bioinformatics 2012 13(Suppl 4):S11 doi:10.1186/1471-2105-13-S4-S11