Histogram of proportions and peak of histogram of proportion normalization. The peak in the histogram corresponds to the largest density of genes. To determine the peak maximum, the histogram was fitted to a beta function. The blue curve shows the best fit with the maximum at pn = 0.527. This is to be compared to the proportion corresponding to RPKM normalization 0.525.
Cai et al. BMC Bioinformatics 2012 13(Suppl 13):S5 doi:10.1186/1471-2105-13-S13-S5