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This article is part of the supplement: UT-ORNL-KBRIN Bioinformatics Summit 2012

Open Access Meeting abstract

The antibiotic resistance proteome of Acinetobacter baumanii MDR isolate MMC#4

Dana Marshall1*, Jianan Dong2, Leon Dent3 and Siddharth Pratap2

Author affiliations

1 Department of Pathology, Anatomy and Cell Biology, Meharry Medical College, Nashville, TN 37208, USA

2 Bioinformatics and Proteomics Core, Meharry Medical College, Nashville, TN 37208, USA

3 Department of Surgery, Meharry Medical College, Nashville, TN 37208, USA

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Citation and License

BMC Bioinformatics 2012, 13(Suppl 12):A20  doi:10.1186/1471-2105-13-S12-A20


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2105/13/S12/A20


Published:31 July 2012

© 2012 Marshall et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Two hundred and forty-seven isolates of Acinetobacter baumanii (AB) were identified in the Nashville General Hospital at Meharry epidemiology database for a three year period. Of these isolates, 77% were multi-drug resistant (MRAB). Mechanical ventilation and multiple site recovery were associated with MRAB, and MRAB isolates were associated with increased mortality relative to sensitive AB isolates [1]. AB acquires resistance rapidly and the mechanisms are still being identified. Proteomic analysis can identify proteins that change in their expression levels in the presence of antibiotics, a possible mechanism of resistance for AB. No proteome databases exist for this organism so antibiotic sensitivity and resistance proteomes were generated for MRAB bronchial wash isolate MMC#4.

Materials and methods

AB MMC#4 was grown in plain LB broth or LB broth supplemented with MIC50 concentrations of levofloxacin, tobramycin, gentamicin, cefotaxime and meropenem. Cell pellets were lysed and total protein run on an SDS-PAGE gel. Protein bands were excised and in-gel digested with trypsin. Resulting peptides were analyzed using a Thermo Finnigan LTQ ion trap mass spectrometer equipped with a 1-D nanoLC pump (Eksigent), Nanospray source (James A Hill Company), and Xcalibur 2.0 SR2 instrument control (Thermo Scientific). Peptides were separated on a packed capillary tip (Polymicro Technologies, 50 μm ID X 9 cm) with C18 reverse phase resin (5 μm, 300 Å, Phenomenex). Tandem spectra were acquired using a data dependent scanning mode in which one full MS scan (m/z 200-2000) was followed by 5 MS-MS scans. Tandem spectra were searched against the NCBI AB strain ACICU database using MryiMatch and IDPicker software. The search database was concatenated with the reverse sequences to determine false discovery rates. Proteins identified by less than two peptide spectral were eliminated (FDR <5%) and the output was filtered using IDPicker using a false positive ID threshold of 5%. Protein reassembly from identified peptide sequences was done as described by Zhang et al. [2].

Results and conclusions

This analysis resulted in the identification of 125 high-confidence hits. Ten of these are presented in Table 1. Antibiotic stress resulted in increased detection of beta-lactamase (cefotaxime is a beta-lactam antibiotic) as well as several proteins associated with oxidative stress that have not previously been described in the context of MRAB resistance mechanisms. These results reinforce the utility of proteomes of antibiotic resistance for MRAB isolates in the identification of potential diagnostic and therapeutic targets, as well as resistance mechanisms, for this emerging pathogen.

Table 1. Top 10 peptides identified more frequently in AB grown in the presence of antibiotic pressure when compared to AB grown in the absence of antibiotic pressure.

Acknowledgements

This research was supported by The Meharry Translational Research Center (MeTRC) Grant Number U54RR026140-01 and The Research Centers in Minority Institutes (RCMI) Grant Number 3G12RR003032-24S1 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). The research was also supported in part by Vanderbilt CTSA grant 1 UL1 RR024975 from the National Center for Research Resources, National Institutes of Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.

References

  1. Dent LL, Marshall DR, Pratap S, Hulette RB: Multidrug resistant Acinetobacter baumanii: A descriptive study in a city hospital.

    BMC Infect Dis 2010, 10:196-202. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  2. Zhang B, Chambers MC, Tabb DL: Proteomic parsimony through bipartite graph analysis improves accuracy and transparency.

    J Proteome Res 2007, 9:3549-3557. OpenURL