Table 2

Examples of producible nonnative metabolites under conditions of maximized biomass production
Nonnative metabolites Host network By-product No. of reaction(s) Heterologous reaction(s) EC number
Pentane-2,4-dione E. coli, S. cerevisiae Oxygen 1 Pentane-2,4-dione + oxygen ↔ acetate + methylglyoxal 1.13.11.50
C. glutamicum Oxygen 2 Glycerone phosphate ↔ methylglyoxal + orthophosphate 4.2.3.3
Pentane-2,4-dione + oxygen ↔ acetate + methylglyoxal 1.13.11.50
Vanillin (4-hydroxy-3-methoxy -benzaldehyde) E. coli, C. glutamicum Oxygen, NADH 4 Formaldehyde + NAD+ + H2O ↔ formate + NADH + H+ 1.2.1.46
3-Dehydroshikimate ↔ 3,4-dihydroxybenzoate + H2O 4.2.1.118
Vanillate + oxygen + NADH + H+ ↔ 3,4-dihydroxybenzoate + NAD + + H2O + formaldehyde 1.14.13.82
Vanillate + NAD+ + H2O ↔ 4-hydroxy-3-methoxy-benzaldehyde  + oxygen + NADH + H+ 1.2.3.9
(R)-Propane-1,2-diol E. coli NAD+ 2 (R)-Lactaldehyde + NAD+ + H2O ↔ (R)-lactate + NADH + H+ 1.2.1.23
(R)-Propane-1,2-diol + NAD+ ↔ (R)-lactaldehyde + NADH + H+ 1.1.1.77
2-Propyn-1-al S. cerevisiae NAD+ 3 3-Oxopropanoate ↔ acetaldehyde + CO2 4.1.1.-
3-Oxopropanoate ↔ propynoate + H2O 4.2.1.27
2-Propyn-1-al + NAD+ + H2O ↔ propynoate + NADH + H+ 1.2.1.3
Adipate semialdehyde E. coli NADP+ 6 Succinyl-CoA + acetyl-CoA ↔ CoA + 3-oxoadipyl-CoA 2.3.1.174
(3 S)-3-Hydroxyadipyl-CoA + NAD+ ↔ 3-Oxoadipyl-CoA + NADH + H+ 1.1.1.35
5-Carboxy-2-pentenoyl-CoA + H2O ↔ (3 S)-3-hydroxyadipyl-CoA 4.2.1.17
Adipyl-CoA + FAD ↔ 5-carboxy-2-pentenoyl-CoA + FADH2 1.3.99.-
Adipate + CoA + ATP ↔ Adipyl-CoA + AMP + diphosphate 6.2.1.-
Adipate semialdehyde + NADP+ + H2O ↔ adipate + NADPH + H+ 1.2.1.4

Chatsurachai et al.

Chatsurachai et al. BMC Bioinformatics 2012 13:93   doi:10.1186/1471-2105-13-93

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