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Open Access Highly Accessed Software

Hydra: a scalable proteomic search engine which utilizes the Hadoop distributed computing framework

Steven Lewis1*, Attila Csordas2, Sarah Killcoyne3, Henning Hermjakob2, Michael R Hoopmann1, Robert L Moritz1, Eric W Deutsch1 and John Boyle1

Author Affiliations

1 Institute for Systems Biology, Seattle, WA, USA

2 PRIDE Group Proteomics Services Team EMBL European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK

3 Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Luxembourg, Germany

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BMC Bioinformatics 2012, 13:324  doi:10.1186/1471-2105-13-324

Published: 5 December 2012

Abstract

Background

For shotgun mass spectrometry based proteomics the most computationally expensive step is in matching the spectra against an increasingly large database of sequences and their post-translational modifications with known masses. Each mass spectrometer can generate data at an astonishingly high rate, and the scope of what is searched for is continually increasing. Therefore solutions for improving our ability to perform these searches are needed.

Results

We present a sequence database search engine that is specifically designed to run efficiently on the Hadoop MapReduce distributed computing framework. The search engine implements the K-score algorithm, generating comparable output for the same input files as the original implementation. The scalability of the system is shown, and the architecture required for the development of such distributed processing is discussed.

Conclusion

The software is scalable in its ability to handle a large peptide database, numerous modifications and large numbers of spectra. Performance scales with the number of processors in the cluster, allowing throughput to expand with the available resources.