Distribution of HPCall base-calling probabilities. The empirical cumulative distribution functions of probabilities estimated by HPCall for sequences with reference HPLref 3. The depicted probabilities are (A) cumulative sum of probabilities below HPL 3, (B) probabilities at HPL 3, and (C) cumulative sum of probabilities above HPL 3. In each panel the cumulative distribution functions are plotted separately in case of an undercall, correct call or overcall. The distributions of probabilities clearly indicate larger base-calling uncertainties in case of an undercall or overcall.
Beuf et al. BMC Bioinformatics 2012 13:303 doi:10.1186/1471-2105-13-303