Distribution of HPCall quality scores at HPLref3 overcall. The empirical cumulative distribution function of HPCall quality scores QSovercall assigned to bases associated with HPL 2, 3 and 4 for sequences with reference HPLref 3. The quality scores associated with HPL 2 and HPL 3 are generally large, whereas HPL 4 gives smaller quality scores. HPCall clearly indicates that overcalls are more likely in this situation, whereas this insight is not provided by the native 454 quality scores.
Beuf et al. BMC Bioinformatics 2012 13:303 doi:10.1186/1471-2105-13-303