The effect of preprocessing raw intensities. Raw intensities and flowgram values versus cycle number for a typical read, illustrating the effect of preprocessing. The colors represent the reference HPL. The preprocessing of raw intensities to flowgram values removes much noise. However, the raw intensities still contain additional information that can be used in the base-calling.
Beuf et al. BMC Bioinformatics 2012 13:303 doi:10.1186/1471-2105-13-303