Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation
1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, DK-5230, Denmark
2 Biomedical Imaging Group, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, CH-1015, Switzerland
3 Institute for Mathematics and Computer Science (IMADA), University of Southern Denmark, Odense M, DK-5230, Denmark
4 Department of Physics, Chemistry and Pharmacy, MEMPHYS Center for Biomembrane Physics, University of Southern Denmark, Odense M, DK-5230, Denmark
BMC Bioinformatics 2012, 13:296 doi:10.1186/1471-2105-13-296Published: 13 November 2012
Additional file 1:
Figure S1. Simulation of the StrExp function and distance-dependence of fitting to homogenous diffusion. Figure S2. Bleach profile of the Argon laser at 488 nm for various objectives. Figure S3. 3D simulation of a FLIP experiment with heterogeneous diffusion in the unit sphere. Figure S4. FLIP experiment of BODIPY-cholesterol in CHO cells and fitting with the StrExp function. Figure S5. Simulation of the homogeneous compartment model and fitting with the StrExp function. Figure S6. Effect of additive image noise on fitting performance. Figure S7. Effect of time noise on fitting performance. Figure S8. Correlation between amplitude and time constant maps in the StrExp fit to the spatially Figure S9. Pixel-wise FLIP analysis of eGFP in the nucleus.heterogeneous model.
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Movie 1. Numerical simulation of FLIP experimentwith space-dependent diffusion coefficient.
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Movie 2. Time course of barrier-limited FLIPexperiment of eGFP.
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