Table 2

The variance in the number of primers obtained from 1–5x redundancy
Redundancy Frequency of redundancy value when maximum Number of primers >0 Average number of primers identified Standard error
5x 14 14 0.01 0
4x 867 881 1.28 0.03
3x 130 1005 4.39 0.05
2x 9 1020 99.54 0.1
1x 0 1020 437.41 0.22

The algorithm was repeated 1020 times with a small subset of 30 sequences. The maximum achieved redundancy obtained by MCMC-ODPR in each run was recorded and when no primers were obtained at a certain level of redundancy, a zero was added as an observation, ensuring observations at all levels of redundancy summed to 1020. Column 2 gives the number of frequency of primers found at each level of redundancy throughout all 1020 runs when their numbers were greater than none. The average number of primers identified by the algorithm each run and the standard error is presented.

Kitchen et al.

Kitchen et al. BMC Bioinformatics 2012 13:287   doi:10.1186/1471-2105-13-287

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