Table 4

Titanium genotypes with more error-containing than Sanger sequence matching reads
Reads of nearby pyrosequence Reads of sanger sequence Errors
5 0 G8→G7 (301),
G5→G4 (354)
70 0 G6→G5 (133)
75 1 C7→C6 (313)
21 18 G6→G5 (133)
14 0 G6→G5 (133)
80 0 G6→G5 (133)
77 3 G6→G5 (133)
80 1 G6→G5 (133)

The difference between the Sanger and 454 sequences and their locations are given. For example, G6→G5 (133) means that a homopolymer beginning at the 133rdbase with 6 Gs in a Sanger sequence showed up as 5 Gs in most of the pyrosequence reads. All disagreements consist of deletions in the 454 reads on long G/C homopolymers relative to the Sanger sequences, and the majority of the disagreements occurred on one locus, site 133.

Rosen et al.

Rosen et al. BMC Bioinformatics 2012 13:283   doi:10.1186/1471-2105-13-283

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