|Titanium genotypes with more error-containing than Sanger sequence matching reads|
|Reads of nearby pyrosequence||Reads of sanger sequence||Errors|
The difference between the Sanger and 454 sequences and their locations are given. For example, G6→G5 (133) means that a homopolymer beginning at the 133rdbase with 6 Gs in a Sanger sequence showed up as 5 Gs in most of the pyrosequence reads. All disagreements consist of deletions in the 454 reads on long G/C homopolymers relative to the Sanger sequences, and the majority of the disagreements occurred on one locus, site 133.
Rosen et al.
Rosen et al. BMC Bioinformatics 2012 13:283 doi:10.1186/1471-2105-13-283