Table 4 |
||
| Genes used in the qRT-PCR assays, and the sequence of the PCR primers used in the assays | ||
| Gene information | Gene role | Forward primer (5` to 3`)Reverse primer (3` to 5`) |
| UBE2Z, NM_023079.3 | Reference gene | GCAGAGCATGTCTGGCATAG TTCTCCTTCTGCCAAAACAAA |
| PGK1, NM_000291.3 | Reference gene | TGCATCTCCACTTGGCATTA TGGGATCTTGAAGAATGTATGC |
| SRRM1, NM_005839.3 | Reference gene | GGAAATCCTTGGGTTTGAAGA GGCCACAGTTCTCCCATAAA |
| COX4I1, NM_001861.2 | Reference gene | GGCACTGAAGGAGAAGGAGA GGGCCGTACACATAGTGCTT |
| B2M, NM_004048 | DE gene determined by LO-BaFL/SAM | GATGAGTATGCCTGCCGTGTG CAATCCAAATGCGGCATCT |
| ACTG1, NM_001614 | DE gene determined by LO-BaFL/SAM | AGAGGCTGGCAAGAACCAGTTGTT CAATGACGTGTTGCTGGGGCCT |
| DYNLT1, NM_006519.1 | DE gene determined by TM4 analysis | CCAGCCTATGGCCTTTCTCCTTTTGT CAACGCAGGCTGCAGGTGAC |
| SKIV2L2, NM_015360.4 | DE gene determined by TM4 analysis | TGCAGAAGGAATCACCAAAA ATGGGAGAACCAAATCCACA |
| C12orf35, NM_018169.3 | DE gene determined by TM4 analysis | CGGGGAAACAAGGTATTTGA TTCACATCACAGTGGGCATT |
| TARDBP, NM_007375.3 | DE gene determined by TM4 analysis | TTTGCTGCAGTTCTGTGTCC TCCATCTCAAAAGGTCAAAA |
| ILKAP, NM_030768.2 | Cross-hybridizing gene with TARDBP | CACAGGAGTACACAAAACACAC TGCGGATAGGGCACTGAG |
Baciu et al. BMC Bioinformatics 2012 13:244 doi:10.1186/1471-2105-13-244
