Examples of potential unintended targets for primer pairs generated by QuantPrime and PRIMEGENS. Example targets are extracted from Primer-BLAST specificity checking results for primer pairs generated by QuantPrime or PRIMEGENS (a total of 162 and 116 potential unintended targets were identified for QuantPrime and PRIMEGENS, respectively. See Table 2 for details). The example primers correspond to those underlined in Additional files 1 and 2.
Ye et al. BMC Bioinformatics 2012 13:134 doi:10.1186/1471-2105-13-134