Figure 5.

Examples of potential unintended targets for primer pairs generated by QuantPrime and PRIMEGENS. Example targets are extracted from Primer-BLAST specificity checking results for primer pairs generated by QuantPrime or PRIMEGENS (a total of 162 and 116 potential unintended targets were identified for QuantPrime and PRIMEGENS, respectively. See Table‚ÄČ2 for details). The example primers correspond to those underlined in Additional files 1 and 2.

Ye et al. BMC Bioinformatics 2012 13:134   doi:10.1186/1471-2105-13-134
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