Additional file 6.
PCR experimental validation of the detected EEJ, NM_021019 (7:2-4). The description of the marker lane and the abbreviations are the same with the ones of Fig. 4c. The forward and reverse primers were located at exon 2 and exon 4, respectively. (a) The PCR result showed that the expected EEJ (d transcript) can be experimentally detected. Besides, the major c and the minor d transcripts, the c alternative transcript (with 108 bp fewer than c transcript) was also detected. Both of the detected c alternative and d transcript have EST evidences. (b) The sequencing result provided the authenticity of the detected EEJs.
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Li et al. BMC Bioinformatics 2011 12(Suppl 1):S9 doi:10.1186/1471-2105-12-S1-S9