Yeast dataset: Read count vs. GC-content. Lowess fits of gene-level log(count + 1) vs. GC-content for the eight YPD lanes from the Yeast dataset, after FQ between-lane normalization. Curves are colored according to culture/library preparation. The GC-content effect is the same for lanes assaying the same culture/library preparation, but can be different for lanes assaying different cultures/library preparations. Figure S4 displays the scatterplot and lowess fit for the first YPD lane (culture/library preparation Y1, flow-cell 428R1).
Risso et al. BMC Bioinformatics 2011 12:480 doi:10.1186/1471-2105-12-480