Table 2

PCR per base error probabilities for the three 'Even' V2 'Mock Communities'

Nucleotide

A

C

T

G

Total f

Per cycle p


A

0.9995

7.2e-6

7.7e-6

5.1e-4

5.2e-4

3.5e-5

C

1.1e-05

0.9996

4.1e-4

2.1e-6

4.2e-4

2.8e-5

T

9.0e-6

5.7e-4

0.9994

1.4e-5

5.9e-4

4.0e-5

G

3.5e-4

3.2e-6

2.1e-5

0.9996

3.7e-4

2.5e-5


The rows give the true nucleotides and the columns the observed as a result of errors. Estimates of the individual probabilities are given as frequencies. The total error probability f for each nucleotide is also quoted in the penultimate column, followed by the per cycle error rate p calculated as p = 2f /n, where n = 30 is the cycle number [8,17].

Quince et al. BMC Bioinformatics 2011 12:38   doi:10.1186/1471-2105-12-38

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