Viral RNAi screens are enriched for host factors that are subunits of human complexes. (A) All viral RNAi screens identify significantly more human complex subunits identified than expected (HIV 23%, influenza 25%, and hepatitis C 24%), compared to the number of proteins in the human genome assigned to complexes by CORUM (12%). P values shown are based on the hypergeometric distribution. We find no strong enrichment of protein complexes in a screen of Mtb host factors (13%). (B) Network of Vif interactors from GPS-Prot using the optional NIAID HIV-1-human interactions database, instead of VirusMINT. Including CORUM as a database brings complex subunits closer together in the network, for example the cluster of proteasome complex subunits shown to the lower left (e.g. PSMA, PSMB, PSMC, etc).
Fahey et al. BMC Bioinformatics 2011 12:298 doi:10.1186/1471-2105-12-298