Multi-species interolog flowchart and PRIN architecture. Protein-protein interaction data of reference organisms (yeast, human, fruitfly, worm, E.coli and A.thaliana) are integrated from public databases. The orthologous protein groups between rice and these reference organisms are clustered by InParanoid algorithm. A mapping process, known as interolog, is used to predict protein-protein interactions. Genomic features of rice are additionally mapped to the predicted interactome, RSSGO scores (see Results and Methods), co-localization and co-expression Pearson correlation coefficient scores are calculated for further network validation and database annotation. Finally a well organized web server PRIN is developed to visualize our network and search database.
Gu et al. BMC Bioinformatics 2011 12:161 doi:10.1186/1471-2105-12-161