A PCR assay for the 16-nt RNA molecule S = UUCGCGCUUUCCCCUG. Two nuclear extracts and two cytoplasmic extracts from hNP1 human neural progenitor cells were assayed for the 16-nt sequence S. The two nuclear Rn values at 17 PCR cycles were about 1.7 versus the two cytoplasmic Rn values about 1.0. (Rn is the ratio of fluorescence emission intensity of the reporter dye divided by the fluorescence emission intensity of a passive reference dye.) Two types of negative controls were not detected in both nuclear and cytoplasmic fractions (see text).
Jeffries et al. BMC Bioinformatics 2010 11:602 doi:10.1186/1471-2105-11-602