Resolution:
## Figure 1.
Scatter plot of the log. A. Scatter plot of the log_{10 }rpm in two samples_{10 }rpm in two samples. The greater spread at lower counts suggests higher variance. B.
Sample variance of log_{10 }rpm from a simulation with constant biological variation shows that the increased
sample variance may be caused by poor approximation of a Gaussian distribution to
a Binomial distribution for sparse counts. To create the simulation dataset, we sampled
log(π_{0}) from observed average log counts and created log(π_{1}) = log(π_{0}) + δ/2 and log(π_{2}) = log(π_{0}) - δ/2 where δ ~ N (0, σ^{2}). In this plot σ = 0.122, as estimated in the example data.
Wu |