Two-dimensional FCM data. 853,674 U937 cells are stained by two florescence dyes, Pacific Blue and APC-Cy7-A. a) The fluorescence intensity of APC-Cy7-A is plotted against the fluorescence intensity of Pacific Blue. b) Result of the cluster analysis by using the Misty Mountain method. Each cluster is marked by a code number. Table in Additional File 2 lists the characteristics of the resulting clusters.
Sugár and Sealfon BMC Bioinformatics 2010 11:502 doi:10.1186/1471-2105-11-502