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Open Access Software

Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing

David-Olivier D Azulay1*, Hendrik Neubert2 and Mireia Fernández Ocaña3

Author Affiliations

1 Molecular Medicine, Pfizer Global Research and Development, Sandwich, UK

2 Pharmacokinetics Dynamics and Metabolism, Pfizer Global Research and Development, Sandwich, UK

3 Structural Biology and Biophysics, Pfizer Global Research and Development, Sandwich, UK

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BMC Bioinformatics 2010, 11:371  doi:10.1186/1471-2105-11-371

Published: 5 July 2010

Abstract

Background

OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality.

Results

We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions.

Conclusions

A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.