Software
Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
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* Corresponding author: David-Olivier D Azulay david-olivier.azulay@pfizer.com
1 Molecular Medicine, Pfizer Global Research and Development, Sandwich, UK
2 Pharmacokinetics Dynamics and Metabolism, Pfizer Global Research and Development, Sandwich, UK
3 Structural Biology and Biophysics, Pfizer Global Research and Development, Sandwich, UK
BMC Bioinformatics 2010, 11:371 doi:10.1186/1471-2105-11-371
Published: 5 July 2010Abstract
Background
OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality.
Results
We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions.
Conclusions
A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.