Methodology article
Shape based kinetic outlier detection in real-time PCR
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* Corresponding authors: Davide Sisti davide.sisti@uniurb.it - Michele Guescini michele.guescini@uniurb.it
- † Equal contributors
1 Dipartimento DiSUAN, Sezione di Biomatematica, Università degli Studi di Urbino "Carlo Bo", Campus Scientifico Sogesta; Località Crocicchia - 61029 Urbino, Italy
2 Dipartimento di Scienze Biomolecolari, Sezione di Ricerca sull'Attività Motoria e della Salute, Università degli Studi di Urbino "Carlo Bo", Via I Maggetti, 26/2 - 61029 Urbino, Italy
BMC Bioinformatics 2010, 11:186 doi:10.1186/1471-2105-11-186
Published: 12 April 2010Additional files
Additional file 1:
Fluorescence data and fitting elaboration of standard sample amplifications (standard curve) and amplifications obtained in the presence of: tannic acid, IgG and quercitin.
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Additional file 2:
Analytical solutions for the y value of the inflection point (Yf.) and the slope of tangent straight-line (m) crossing the inflection point.
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Additional file 3:
A) Chi-square distribution of the squared distances about the population mean vector (D2 = (y-μ)'Σ-1(y-μ)) with 3 degrees of freedom. B) Scatter plots of all pairs of variables Fmax, Yf and m.
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Additional file 4:
P-P plot of the variable Log(Nob/Nexp).
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Additional file 5:
KOD and SOD elaborations of standard sample amplifications (standard curve) and amplifications obtained in the presence of: tannic acid, IgG and quercitin.
Format: XLS Size: 209KB Download file
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