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Open Access Highly Accessed Software

web cellHTS2: A web-application for the analysis of high-throughput screening data

Oliver Pelz, Moritz Gilsdorf and Michael Boutros*

Author Affiliations

German Cancer Research Center (DKFZ), Div Signaling and Functional Genomics and University of Heidelberg, Faculty of Medicine Mannheim, Dept Cell and Molecular Biology, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany

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BMC Bioinformatics 2010, 11:185  doi:10.1186/1471-2105-11-185

Published: 12 April 2010

Abstract

Background

The analysis of high-throughput screening data sets is an expanding field in bioinformatics. High-throughput screens by RNAi generate large primary data sets which need to be analyzed and annotated to identify relevant phenotypic hits. Large-scale RNAi screens are frequently used to identify novel factors that influence a broad range of cellular processes, including signaling pathway activity, cell proliferation, and host cell infection. Here, we present a web-based application utility for the end-to-end analysis of large cell-based screening experiments by cellHTS2.

Results

The software guides the user through the configuration steps that are required for the analysis of single or multi-channel experiments. The web-application provides options for various standardization and normalization methods, annotation of data sets and a comprehensive HTML report of the screening data analysis, including a ranked hit list. Sessions can be saved and restored for later re-analysis. The web frontend for the cellHTS2 R/Bioconductor package interacts with it through an R-server implementation that enables highly parallel analysis of screening data sets. web cellHTS2 further provides a file import and configuration module for common file formats.

Conclusions

The implemented web-application facilitates the analysis of high-throughput data sets and provides a user-friendly interface. web cellHTS2 is accessible online at http://web-cellHTS2.dkfz.de webcite. A standalone version as a virtual appliance and source code for platforms supporting Java 1.5.0 can be downloaded from the web cellHTS2 page. web cellHTS2 is freely distributed under GPL.