Figure 3.

Diagram showing the experimental validation of the multiplex PCR by using the primer set combination (PSC) designed by MPprimer for five mouse genes (β-actin, B2m, Pgk1, GAPDH and Rp113a). The top panel showing the predicted (virtual) electrophoretogram of five conventional PCRs and a multiplex PCR by MPprimer. The middle and bottom panels showing the experimentally validated electrophoretogram. The plus sign indicates the use of the primer set.