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This article is part of the supplement: UT-ORNL-KBRIN Bioinformatics Summit 2009

Open Access Meeting abstract

Reconstructing the virulome of the human pathogen Streptococcus pyogenes using NMPDR subsystems-based annotation

Ramy K Aziz12* and Leslie K Mcneil3

Author Affiliations

1 Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt

2 Computation Institute, University of Chicago, Chicago, IL, USA

3 National Center for Supercomputing Applications, University of Illinois, Urbana, IL, USA

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BMC Bioinformatics 2009, 10(Suppl 7):A7  doi:10.1186/1471-2105-10-S7-A7

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2105/10/S7/A7


Published:25 June 2009

© 2009 Aziz and Mcneil; licensee BioMed Central Ltd.

Background

The increasing number of published complete microbial genomes has revolutionized biological sciences and is driving a paradigm shift in microbiology. While this genomic revolution has made the reconstruction of an organism's metabolism from genomic data achievable, predicting the pathogenic potential of host-associated microbes is still in its early stages; hence, developing innovative bioinformatics tools that integrate microbiologists' expertise and experimental laboratory data with sequence data remains a necessity. For this purpose, the NIH-funded National Microbial Pathogen Data Resource (NMPDR, http://www.nmpdr.org webcite) was established as a bioinformatics resource center for specific bacterial pathogens, including staphylococci, streptococci, and sexually transmitted bacteria [1]. Genomes in NMPDR are annotated by the recently developed subsystems annotation technology [2,3], available from the SEED environment http://theseed.uchicago.edu/FIG/index.cgi webcite. This technology relies on analyzing genes in their chromosomal context and combines the accuracy of human curation with the speed of automated propagation [2].

Methods and results

In this study, we apply the subsystems annotation technology to reconstruct the virulome of the human pathogen Streptococcus pyogenes that claims 500,000 lives every year [4], and causes a wide range of diseases that affect adults and children [5]. In particular, we use NMPDR tools for pathogenomic comparison of the fully sequenced streptococcal serotypes, and highlight the impact of prophages and highly recombinatorial genomic segments, including the newly discovered pilus locus [6], on streptococcal strain emergence and diversification [7]. Our analysis defines the core and dispensable elements of the streptococcal virulome, which includes – in addition to the ancestral, species-specific virulence proteins – the phage-encoded toxins and their pseudogenes. Finally, we use comparative analysis of streptococcal subsystems in context of actual transcriptome data to gain insight into the complex gene regulatory networks that control virulence.

References

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