Figure 1.

Pipeline of bisulfite sequencing. 1) Denaturation: separating Watson and Crick strands; 2) Bisulfite treatment: converting un-methylated cytosines (blue) to uracils; methylated cytosines (red) remain unchanged; 3) PCR amplification of bisulfite-treated sequences resulting in four distinct strands: Bisulfite Watson (BSW), bisulfite Crick (BSC), reverse complement of BSW (BSWR), and reverse complement of BSC (BSCR).

Xi and Li BMC Bioinformatics 2009 10:232   doi:10.1186/1471-2105-10-232
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