Automated FingerPrint Background removal: FPB
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* Corresponding author: Simone Scalabrin scalabrin@appliedgenomics.org
1 Istituto di Genomica Applicata (IGA), via J. Linussio 51, I-33100 Udine, Italy
2 Department of Mathematics and Computer Science, University of Udine, via delle Scienze 206, I-33100 Udine, Italy
3 Department of Agriculture and Environmental Sciences, University of Udine, via delle Scienze 208, I-33100 Udine, Italy
BMC Bioinformatics 2009, 10:127 doi:10.1186/1471-2105-10-127
Published: 30 April 2009Additional files
Additional file 1:
Particular cases of peaks to be removed. Partially digested fragments, star activity by-products, "machine" background, and E. coli peaks present particular features and need to be removed accordingly.
Format: PDF Size: 193KB Download file
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Additional file 2:
Distribution of true peaks vs background peaks. True and background peaks distribute differently from clone to clone and from dye to dye. Therefore, different scenarios may arise and particular care in background removal should be used.
Format: PDF Size: 108KB Download file
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