Expansion of the ORD enhances fiber formation in the presence of the denaturant urea. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Fiber formation of chimeric proteins SP5NM, SP8NM, SP11NM and SP14NM in (A) 0.5 M Urea FFB (B) 1 M Urea FFB is shown. Data in the graphs are representative of two experiments.
Kalastavadi and True BMC Biochemistry 2008 9:7 doi:10.1186/1471-2091-9-7