Fiber formation of ORE chimeras is faster than that with the wild type ORD of PrP. Recombinant protein was diluted from denaturant (120 ×) to 2.5 μM and fiber formation was followed by Th-T fluorescence. Graphs are plotted as %RFU (Relative fluorescence units) versus time. (A) Sup35NM unseeded (solid line) and Sup35NM seeded with sonicated preformed fibers (dotted line). Reaction was seeded with 2.5% (w/w) of preformed sonicated fibers. (B) Unseeded reactions Sup35NM (solid line), SP5NM (dotted line), SP8NM (short dashed line), SP11NM (dotted and dashed line) and SP14NM (long dashed line).
Kalastavadi and True BMC Biochemistry 2008 9:7 doi:10.1186/1471-2091-9-7