Additional file 1.

Supplementary Figure 1. Identification of novel rat liver nuclear PP1 binding and complex proteins by displacement affinity chromatography. Protein was extracted from isolated rat liver nuclei, incubated with microcystin-Sepharose, the matrix washed extensively and eluted with GKKRVRWADLE peptide, followed by elution with 3 M NaSCN [31]. GKKRVRWADLE and NaSCN eluted samples were concentrated separately to an equal volume and run on 10% SDS-PAGE and stained with Collodial blue. In a parallel experiment, the bands shown above were excised, trypsin digested and analyzed by mass spectrometry for identification. The top matched identified proteins for each band(s) are indicated to the left of the figure. Additional matches for each excised band and details of protein identifications are in Additional file 2 online.

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Moorhead et al. BMC Biochemistry 2008 9:28   doi:10.1186/1471-2091-9-28