Biochemical characterization of recombinant renin. a. The pH rate profile of the hydrolysis of DABCYL-γAbu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS with recombinant human renin. The optimal pH is around pH 6.5 with a poorly defined shoulder at pH 8. b. Full progress curve of the cleavage of fluorogenic substrate with recombinant human renin. The enzyme (1 nM) was incubated at 37°C with 1 μM DABCYL-γAbu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS ([S]<<KM). c. Inhibition of recombinant human renin by standard inhibitors. The percent inhibition is plotted as a function of inhibitor concentration for aliskiren (▼), SR 42128 (○) and pepstatin (●). The line through the data is the least squares fit to a four parameter equation describing inhibition, and the IC50 values determined are 0.8 nM (aliskiren), 4 nM (SR 42128) and 31 μM (pepstatin A), respectively.
Wu et al. BMC Biochemistry 2008 9:19 doi:10.1186/1471-2091-9-19