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Effects of assay temperature and glucose on the S0.5 AMP for bound AMPD from skeletal muscle of control versus frozen wood frogs. |
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| S0.5 AMP (mM) |
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| Control |
Frozen |
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| 25°C, no glucose |
0.46 ± 0.03 |
1.50 ± 0.19 |
| 25°C, 250 mM glucose |
0.30 ± 0.04 a |
0.24 ± 0.03 a |
| 5°C, no glucose |
0.70 ± 0.03 a |
1.77 ± 0.03 a |
| 5°C, 250 mM glucose |
0.87 ± 0.04 a |
2.14 ± 0.04 a |
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Data are means ± SEM, for determinations on Sephadex G50-filtered muscle extracts from n = 4 individual frogs. a – Significantly different from the corresponding value at 25°C without glucose as assessed by the Student's t-test, P < 0.05. | ||
Dieni and Storey BMC Biochemistry 2008 9:12 doi:10.1186/1471-2091-9-12 |
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