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AMPD maximal activity (Vmax) and partitioning between free and bound forms in skeletal muscle from control (5°C-acclimated) and 24 h frozen frogs. |
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| Vmax (U/gww) |
% of total activity |
|
|
|
||
| Control |
||
| Free AMPD |
17.6 ± 1.53 |
79.7 ± 6.46 |
| Bound AMPD |
4.5 ± 0.03 |
20.3 ± 0.13 |
| Total |
22.1 ± 1.56 |
100.0 |
|
|
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| Frozen |
||
| Free AMPD |
17.8 ± 0.69 |
64.6 ± 2.07 a |
| Bound AMPD |
9.8 ± 0.03 a |
35.4 ± 0.09 a |
| Total |
27.6 ± 0.72 a |
100.0 |
|
Data are expressed as units per gram wet weight (U/gww), means ± SEM, for determinations on muscle preparations from n = 8 individual frogs. a Significantly different from the corresponding value in control muscle by the Student's t-test, P < 0.05; percentage data were evaluated after arcsine √ transformation of the raw data. | ||
Dieni and Storey BMC Biochemistry 2008 9:12 doi:10.1186/1471-2091-9-12 |
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